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Or read below about HPLC Systems
Timberline Instruments produces column ovens and a variety of mobile phase heaters for use in liquid chromatography systems
High-Performance Liquid Chromatography (HPLC), Ultra High-Performance Liquid Chromatography (UHPLC) or what is commonly just called LC (liquid Chromatography) is a sophisticated method to separate chemical/biological components dissolved in liquid. There a large number of possibilities in which LC systems may be configured. The following diagrams illustrate how typical (generic) HPLC systems may be configured, explain what components are incorporated, and specifically how Timberline column ovens and mobile phase heaters can be employed.
Analytical Scale
The following diagram shows typical components in an Analytical Scale HPLC system. At Analytical scale, the flow rate of mobile phase is quite low, typically less than 3 ml/min. At analytical scale, the operational temperature of the column (and the temperature associated separation efficiency) is greatly a function of the environmental temperature of the column. Major components shown in the diagram are:
Analytical Scale – with Integral Passive Mobile Phase Heater
The following diagram below includes a passive mobile phase heater built-in to the TL105 ASX Column Heater. The passive phase heater is a heat exchanger bolted to the floor of the column oven. This device heats and stabilizes the temperature of the incoming mobile phase. These heat exchangers are effective at low flows (< 5 ml/min).
Semi-Prep Scale
The following diagram below includes a system that features an integrated active mobile phase heater in the TL105 ASX. This configuration supports mobile phase flow rates up to 35 ml/nim @ 100 Deg C or as much as 50 ml/min @ 55 Deg C. Flow capacity is highly dependent operating temperature. Major components shown in the diagram are:
Semi-Prep to Low Prep Scale
The diagram below depicts a system that features an external, high capacity mobile phase heater. This configuration supports mobile phase flow rates up to 580 ml/nim @ 100 Deg C or as much as 780 ml/min @ 55 Deg C. Flow capacity is highly dependent operating temperature. Major components shown in the diagram are:
